Laboratory of Neuroglial Interactions

We are interested in the mechanisms of interactions between neurons and oligodendrocyte lineage cells under different physiological and pathological conditions, especially in epilepsy. Neuronal activity can regulate myelin formation by oligodendrocytes which might have a profound feedback effect on the patterns of activity in neuronal circuits. Many pathologies of the central nervous system are accompanied by changes in myelin content in the nervous tissue, including epilepsy, where hypomyelination is usually described. This is in contrast to the expected activity-dependent myelination. Our aim is to find out how pathological synchronized neuronal activity in epilepsy affects the function of oligodendrocyte lineage cells and what is the cause of hypomyelination in epilepsy.

We are using a mouse model of focal cortical dysplasia and our experimental approaches include immunohistochemistry, single cell transcriptomics, electrophysiology (patch clamp) and calcium imaging in acute brain slices in combination with in vivo 2-photon imaging.

Research areas

  1. Myelination in epilepsy – the aim of the project is to uncover the mechanisms of hypomyelination in focal cortical dysplasia (project run by Bohdana Hrušková)
  2. Synaptic and non-synaptic communication between neurons and oligodendrocyte precursor cells in epilepsy (project run by Adam Bogdanovič)
  3. Mechanisms of epileptogenesis and ictogenesis in focal cortical dysplasia type I and MOGHE (Mild malformation of cortical development with oligodendroglial hyperplasia in epilepsy) (project run by Nikola Vršková)
  4. The impact of neurosteroids on neuronal hyperactivity – joint project with Dr. Kudová’s group at Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences

Current grant projects

The role of disrupted glial cell functions in the pathogenesis of neurodevelopmental and neurodegenerative diseases – National Institute for Neurology Research, Workpackage 3.2 (2022-2025)

Mechanism of oligodendrocyte lineage cells loss in focal cortical dysplasia – Grant Agency of Charles University – main investigator – Adam Bogdanovič (2024-2026)

International cooperation

Prof. Thóra Káradóttir group, University of Cambridge, UK, and University of Iceland, Reykjavik

Keywords

oligodendrocyte precursor cells, oligodendrocytes, myelin, glial cells, epilepsy, focal cortical dysplasia, patch-clamp, calcium imaging, ion channels, glutamate receptors