Dr. Bohdana Hruskova, PhD

Senior Scientist

bohdana.hruskova@lfmotol.cuni.cz

Department of Physiology, Second Faculty of Medicine, Charles University

Scientific background

With a foundations in molecular biology from Charles University, I investigated the cellular signaling during my diploma thesis, focusing on the interplay between inhibitory G protein-coupled receptors and G proteins. I then continued my PhD studies at the Institute of Experimental Medicine, Academy of Sciences, where I explored inhibition and inhibitory receptors within the mammalian auditory pathway using in vivo electrophysiology and patch clamp. Currently, I am a member of the Laboratory of Neuroglial Interactions at the Department of Physiology, Second Faculty of Medicine, Charles University, where we study epilepsy and attempt to unravel the changes in glial cell behavior that occur during the course of this disease as well as their causes, in order to contribute to the elucidation of the pathogenesis of epilepsy.

Research interests

I am currently investigating the impact of epileptic seizures and somatic mutations within the mTOR signaling cascade on fundamental processes such as proliferation of oligodendrocyte lineage cells, their differentiation and myelination. Using techniques such as immunohistochemistry, electrophysiology and in vivo imaging, I aim to unravel the complex mechanisms underlying the disrupted patterns of myelination observed in epilepsy, ultimately contributing to a deeper understanding of the pathophysiology of this disease and identifying potential therapeutic targets to mitigate its impact on neural circuits.

Selected publications

Hruskova B, Trojanova J, Kralikova M, Melichar A, Suchankova S, Bartosova J, Burianova JS, Popelar J, Syka J, Turecek R. Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body. Neurosci Lett. 2019 Apr 23;699:145-150. https://doi.org/10.1016/j.neulet.2019.01.058

Wang T, Rusu SI, Hruskova B, Turecek R, Borst JG. Modulation of synaptic depression of the calyx of Held synapse by GABA(B) receptors and spontaneous activity. J Physiol. 2013 Oct 1;591(19):4877-94. https://doi.org/10.1113/jphysiol.2013.256875

Hruskova B, Trojanova J, Kulik A, Kralikova M, Pysanenko K, Bures Z, Syka J, Trussell LO, Turecek R. Differential distribution of glycine receptor subtypes at the rat calyx of Held synapse. J Neurosci. 2012 Nov 21;32(47):17012-24. https://doi.org/10.1523/JNEUROSCI.1547-12.2012