Mgr. Karolína Liška, Ph.D.

liska.krln@gmail.com

Ústav fyziologie, 2. LF UK

Pathophysiology of SUDEP

Team leader

Mgr. Karolína Liška, PhD. is an early-career researcher at the Department of Physiology, Second Faculty of Medicine, Charles University. She received her PhD. in neuroscience in which she studied the role of circadian regulation of animal physiology, including brain regions involved in learning and memory. She gained expertise in both in vivo and in vitro techniques, immunohistochemical and molecular biology methods in the process. A highly productive collaboration with the team of Prof. Martin Ralph at the University of Toronto 40 was also coordinated by her. She also undertook an internship at the laboratory of Prof. Jihwan Myung at Taipei Medical University in Taiwan. Here she gained valuable experience with cutting-edge in vitro techniques. Since then, she joined the Department of Physiology after the completion of her PhD. Here she has gained expertise with microsurgery and recording physiological functions in vivo in a chronic epilepsy model. The acquired data required implementation of signal processing, for which she set up a successful collaboration with mathematicians and biomedical engineers. She also coordinates the clinical part of the SUDEP project with neurologists from Motol hospital Epilepsy Center, which involves the analysis of cardiovascular and respiratory functions in people with drug-refractory epilepsy who undergo video-EEG monitoring.

Research interests

In her research, Mgr. Karolína Liška, PhD. focuses on the underlying mechanisms of epilepsy. Her area of particular interest is SUDEP (Sudden Unexpected Death in Epilepsy). This devastating phenomenon occurs unexpectedly in some epilepsy patients, and the aim is to uncover the underlying mechanisms and identify potential biomarkers that could predict its occurrence. To address this, she explores the effects seizures have on vital physiological functions, such as the cardiac and respiratory function and its modulation. By understanding the factors contributing to SUDEP, she hopes to develop preventive strategies and potential therapeutic interventions improve patient outcomes.

The most important publications

Liška, K., Dočkal, T., Houdek, P., Sládek, M., Lužná, V., Semenovykh, K., … & Sumová, A. (2023). Lithium affects the circadian clock in the choroid plexus–A new role for an old mechanism. Biomedicine & Pharmacotherapy, 159, 114292. DOI: 10.1016/j.biopha.2023.114292

Liška, K., Sladek, M., Houdek, P., Shrestha, N., Lužná, V., Ralph, M. R., & Sumova, A. (2022). High Sensitivity of the Circadian Clock in the Hippocampal Dentate Gyrus to Glucocorticoid-and GSK3-Beta-Dependent Signals. Neuroendocrinology, 112(4), 384-398. DOI: 10.1159/000517689

Liška, K., Sládek, M., Čečmanová, V., & Sumová, A. (2021). Glucocorticoids reset circadian clock in choroid plexus via period genes. J Endocrinol, 248, 155-66. DOI: 10.1530/joe-20-0526

Sládek, M., Liška, K., Houdek, P., & Sumová, A. (2021). Modulation of single cell circadian response to NMDA by diacylglycerol lipase inhibition reveals a role of endocannabinoids in light entrainment of the suprachiasmatic nucleus. Neuropharmacology, 185, 108455. DOI: 10.1016/j.neuropharm.2021.108455

Čečmanová, V., Houdek, P., Šuchmanová, K., Sládek, M., & Sumová, A. (2019). Development and entrainment of the fetal clock in the suprachiasmatic nuclei: the role of glucocorticoids. Journal of Biological Rhythms, 34(3), 307-322. DOI: 10.1177/0748730419835360

Researcher ID

0000-0002-7228-6656