Ing. Jan Kudláček DiS., Ph.D.

kudlaceksystem@gmail.com

Ústav fyziologie 2. LF UK

Experimental Neurophysiology Group

Team leader

During my studies at the Faculty of Electrical Engineering of the Czech Technical University, I was mainly involved in audio technology and signal processing. After completing my master’s degree I decided to pursue Ph.D. studies. Among several options, Prof. Přemysl Jiruška sparkled my interest for epilepsy. I learned to work with laboratory rodents and mastered in vivo electrophysiology. I also improved my skills in signal analysis in Matlab. In my doctoral thesis, I investigated the long-term dynamics of epileptic seizures. After completing my PhD, I went to the University of Lund for a year where I learned patch-clamp (single neuron recording), optogenetics and chemogenetics. Currently, I am the leader of the Experimental Neurophysiology Group at the Institute of Physiology, 2nd Faculty of Medicine, Charles University.

Research interests

I am now mainly studying slow fluctuations in seizure risk. These fluctuations have a period of days, weeks or months and occur in most patients with epilepsy and also in animal models of epilepsy. Fluctuations in seizure risk have been described to be intertwined with cyclic changes in interictal EEG signal. Animal models of epilepsy offer the possibility of more detailed monitoring of slow cycles than is possible in patients. Therefore, we study the occurrence and characteristics of seizures, the interictal EEG signal and also some behavioral features using automated video processing. The goal is to find a way to forecast seizure risk.

In addition, I am studying the cellular mechanisms of seizure generation in focal cortical dysplasia (FCD). Using optogenetics and chemogenetics, we are trying to trigger or suppress the activity of different types of nerve cells and to analyze the effect of these manipulations on epileptic activity from electrophysiological recordings. I believe that our findings will contribute to the development of gene therapy for epilepsy.

Although I enjoy participating in experimental work in the lab, most of my time is spent at the computer creating measurement protocols and analyzing measured data.

The most important publications

doi:10.1016/j.nbd.2023.106383
doi:10.1016/j.jneumeth.2024.110126
doi:10.1016/j.nbd.2021.105347
doi:10.1038/s41593-018-0278-y
doi:10.3390/ijms222413243